Evaluation of the protection of primates transfused with variant Creutzfeldt-Jakob disease–infected blood products filtered with prion removal devices: a 5-year update
Original Research
Evaluation of the protection of primates transfused with variant
Creutzfeldt-Jakob disease–infected blood products filtered with prion removal
devices: a 5-year update
Authors
Nathalie Lescoutra-Etchegaray, Nina Jaffré, Chryslain Sumian, Valérie
Durand, Evelyne Correia, Jacqueline Mikol, Sophie Luccantoni-Freire, Audrey
Culeux, Jean-Philippe Deslys, Emmanuel E. Comoy
First published: 3 February
DOI: 10.1111/trf.12999View/save
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This work was supported by a grant from Agence Nationale de la Recherche
(ANR), Project PRIONSECUR ANR05PRIB02302.
Abstract
BACKGROUND
Analysis of archived appendix samples reveals that one in 2000 individuals
in the United Kingdom may carry the infectious prion protein associated with
variant Creutzfeldt-Jakob disease (vCJD), raising questions about the risk of
transfusion transmission from apparently healthy carriers. Blood leukoreduction
shows limited efficiency against prions. Therefore, in absence of antemortem
diagnostic tests, prion removal filters, including the P-Capt filter were
designed to improve blood transfusion safety.
STUDY DESIGN AND METHODS
We evaluated the performances of two filters, the P-Capt and one prototype
(PMC#005), with blood-borne infectivity in two independent experiments. Blood
was drawn twice from prion-infected macaques. Corresponding RBCCs were prepared
according to two different procedures: in Study A, the leukoreduction step was
followed by the filtration through the P-Capt. In Study B, the leukoreduction
and prion removal were performed simultaneously through the PMC#005. For each
study, two groups of three animals were transfused twice with samples before or
after filtration.
RESULTS
Among the six macaques transfused with nonfiltered samples, five developed
neurologic signs but only four exhibited peripheral detectable
protease-resistant prion protein (PrPres) accumulation. In Study A, the three
animals transfused with P-Capt–filtered samples remain asymptomatic and devoid
of PrPres in lymph node biopsies 6 years after the transfusion. In Study B, one
animal transfused with PMC#005-filtered samples developed vCJD.
CONCLUSION
After 5 to 6 years of progress, this ongoing study provides encouraging
results on the prion blood removal performances of the P-Capt filter in
macaques, an utmost relevant model for human prion diseases.
Wednesday, July 23, 2014
After the storm? UK blood safety and the risk of variant Creutzfeldt-Jakob
Disease
please remember, all iatrogenic CJD is, is sporadic CJD, until route and
source of the iatrogenic event i.e. route and source is documented, and then put
into the public domain. this very seldom happens. ...
Thursday, January 22, 2015
Transmission properties of atypical Creutzfeldt-Jakob disease: a clue to
disease etiology?
Friday, January 10, 2014
vpspr, sgss, sffi, TSE, an iatrogenic by-product of gss, ffi, familial type
prion disease, what it ???
Thursday, January 15, 2015
41-year-old Navy Commander with sporadic Creutzfeldt–Jakob disease CJD TSE
Prion: Case Report
Saturday, January 17, 2015
*** Becky Lockhart 46, Utah’s first female House speaker, dies diagnosed
with the extremely rare Creutzfeldt-Jakob disease
Tuesday, December 30, 2014
TSEAC USA Reason For Recalls Blood products, collected from a donors
considered to be at increased risk for Creutzfeldt-Jakob Disease (CJD), were
distributed END OF YEAR REPORT 2014
*** HUMAN MAD COW DISEASE nvCJD TEXAS CASE NOT LINKED TO EUROPEAN TRAVEL
CDC ***
Sunday, November 23, 2014
*** Confirmed Variant Creutzfeldt-Jakob Disease (variant CJD) Case in Texas
in June 2014 confirmed as USA case NOT European ***
the patient had resided in Kuwait, Russia and Lebanon. The completed
investigation did not support the patient's having had extended travel to
European countries, including the United Kingdom, or travel to Saudi Arabia. The
specific overseas country where this patient’s infection occurred is less clear
largely because the investigation did not definitely link him to a country where
other known vCJD cases likely had been infected.
Sunday, December 14, 2014
*** ALERT new variant Creutzfeldt Jakob Disease nvCJD or vCJD, sporadic CJD
strains, TSE prion aka Mad Cow Disease United States of America Update December
14, 2014 Report ***
price of prion poker goes up again with this study. I strongly urge the
United States FDA et al to revisit their failed ruminant mad cow feed ban, where
still to this day, the feed ban does NOT include cervids. ...
Saturday, January 31, 2015
European red deer (Cervus elaphus elaphus) are susceptible to Bovine
Spongiform Encephalopathy BSE by Oral Alimentary route
Saturday, January 31, 2015
RAPID ADVICE 17-2014 : Evaluation of the risk for public health of casings
in countries with a “negligible risk status for BSE” and on the risk of
modification of the list of specified risk materials (SRM) with regard to BSE
Saturday, January 24, 2015
Bovine Spongiform Encephalopathy: Atypical Pros and Cons
Discussion: The C, L and H type BSE cases in Canada exhibit molecular
characteristics similar to those described for classical and atypical BSE cases
from Europe and Japan. *** This supports the theory that the importation of BSE
contaminated feedstuff is the source of C-type BSE in Canada. *** It also
suggests a similar cause or source for atypical BSE in these countries. ***
see page 176 of 201 pages...tss
*** Singeltary reply ; Molecular, Biochemical and Genetic Characteristics
of BSE in Canada Singeltary reply ;
ruminant feed ban for cervids in the United States ?
31 Jan 2015 at 20:14 GMT
Self-Propagative Replication of Ab Oligomers Suggests Potential
Transmissibility in Alzheimer Disease
Received July 24, 2014; Accepted September 16, 2014; Published November 3,
2014
Singeltary comment ;
re-Human Prion Diseases in the United States
Posted by flounder on 01 Jan 2010 at 18:11 GMT
TSS
posted by Terry S. Singeltary Sr. at
9:29 PM
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