Cleaning, disinfection and sterilization of surface prion contamination
Article in Press
Cleaning, disinfection and sterilization of surface prion contamination
G. McDonnell Affiliations STERIS Corporation, Mentor, Ohio, USA
Corresponding Author InformationCorresponding author. Address: STERIS
Corporation, 5960 Heisley Road, Mentor, OH 44060, USA. Tel.: +1 440 392 7118.
email address, C. Dehen Affiliations CEA, Institute of Emerging Diseases and
Innovative Therapies (iMETI), Division of Prions and Related Diseases (SEPIA),
Fontenay-aux-Roses, France , A. Perrin Affiliations CEA, Institute of Emerging
Diseases and Innovative Therapies (iMETI), Division of Prions and Related
Diseases (SEPIA), Fontenay-aux-Roses, France , V. Thomas Affiliations STERIS,
CEA, Fontenay-aux-Roses, France , A. Igel-Egalon Affiliations CEA, Institute of
Emerging Diseases and Innovative Therapies (iMETI), Division of Prions and
Related Diseases (SEPIA), Fontenay-aux-Roses, France STERIS, CEA,
Fontenay-aux-Roses, France , P.A. Burke Affiliations STERIS Corporation, Mentor,
Ohio, USA , J.P. Deslys Affiliations CEA, Institute of Emerging Diseases and
Innovative Therapies (iMETI), Division of Prions and Related Diseases (SEPIA),
Fontenay-aux-Roses, France , E. Comoy Affiliations CEA, Institute of Emerging
Diseases and Innovative Therapies (iMETI), Division of Prions and Related
Diseases (SEPIA), Fontenay-aux-Roses, France
Received 3 October 2012; accepted 27 August 2013. published online 25
September 2013. Accepted Manuscript
Abstract PDF
Summary Background
Prion contamination is a risk during device reprocessing, being difficult
to remove and inactivate. Little is known of the combined effects of cleaning,
disinfection and sterilization during a typical reprocessing cycle in clinical
practice.
Aim
To investigate the combination of cleaning, disinfection and/or
sterilization on reducing the risk of surface prion contamination.
Methods
In vivo test methods were used to study the impact of cleaning alone and
cleaning combined with thermal disinfection and high- or low-temperature
sterilization processes. A standardized test method, based on contamination of
stainless steel wires with high titres of scrapie-infected brain homogenates,
was used to determine infectivity reduction.
Findings
Traditional chemical methods of surface decontamination against prions were
confirmed to be effective, but extended steam sterilization was more variable.
Steam sterilization alone reduced the risk of prion contamination under normal
or extended exposure conditions, but did show significant variation. Thermal
disinfection had no impact in these studies. Cleaning with certain defined
formulations in combination with steam sterilization can be an effective prion
decontamination process, in particular with alkaline formulations.
Low-temperature, gaseous hydrogen peroxide sterilization was also confirmed to
reduce infectivity in the presence and absence of cleaning.
Conclusion
Prion decontamination is affected by the full reprocessing cycle used on
contaminated surfaces. The correct use of defined cleaning, disinfection and
sterilization methods as tested in this report in the scrapie infectivity assay
can provide a standard precaution against prion contamination.
Keywords: Cleaning, Disinfection, Prion, Reprocessing, Sterilization
Sunday, September 08, 2013
Iatrogenic Creutzfeldt-Jakob disease via surgical instruments and
decontamination possibilities for the TSE prion
Friday, August 16, 2013
*** Creutzfeldt-Jakob disease (CJD) biannual update August 2013 U.K. and
Contaminated blood products induce a highly atypical prion disease devoid of
PrPres in primates
Sunday, August 11, 2013
Creutzfeldt-Jakob Disease CJD cases rising North America updated report
August 2013
*** Creutzfeldt-Jakob Disease CJD cases rising North America with Canada
seeing an extreme increase of 48% between 2008 and 2010
Tuesday, September 17, 2013
USAHA 116TH ANNUAL MEETING October 18 – 24, 2012 CWD, Scrapie, BSE, TSE
prion (September 17, 2013)
http://creativegumbo.net/cjdvoice/
kind regards, terry
posted by Terry S. Singeltary Sr. at
10:49 AM
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