Epidemiological and Clinical Characteristics of Sporadic Creutzfeldt–Jakob Disease: A Retrospective Study in Eastern China
Shuo Feng1, Xinjing Zhao2, Xueying Zhou3, 
Xiang Ye2, Xiaolin Yu2, Wei Jiang2, 
Yu Deng4, Shengnian Zhou1, Lin Ma2, Peiyan Shan2 and 
Guoyu Zhou2*- 1Department of Neurology, Qilu Hospital of Shandong University, Jinan, China
- 2Department of Geriatric Neurology, Qilu Hospital of Shandong University, Jinan, China
- 3Department of Physical Medicine and Rehabilitation, Qilu Hospital of Shandong University, Jinan, China
- 4Department of Environmental Health, School of Public Health, China Medical University, Shenyang, China
Objective: We aimed to characterize the epidemiological and clinical characteristics of sporadic Creutzfeldt–Jakob disease (sCJD) in eastern China in this retrospective study.
Methods: This study enrolled 67 patients with sCJD hospitalized in a grade-A tertiary hospital in eastern China from January 2010 to January 2020. Demographic data, clinical symptoms, brain magnetic resonance imaging (MRI), electroencephalogram (EEG), cerebrospinal fluid (CSF) 14-3-3 protein test, polymerase chain reaction (PCR), and DNA sequence determination of genes were collected and analyzed.
Results: There were 62 patients with probable sCJD and 5 patients with possible sCJD. Male (28 cases) to female (39 cases) ratio was 1:1.39. Mean age at disease onset was 64.42 ± 9.00 years (range: 29–88 years), and mean survival time was 9.39 ± 12.58 months (range: 1–60 months for patients who received the follow-ups). The most common onset symptoms were dementia (49.25%), movement disorder (44.78%), and visual disturbance (22.39%), while the most frequent clinical manifestations were language disorders (74.63%), ataxia (70.15%), and myoclonus (70.15%). The positive rates of brain MRI abnormalities, 14-3-3 protein in CSF, and periodic sharp wave complexes (PSWCs) on EEG were 84.90, 68.00, and 46.03%, respectively. The 14-3-3 protein positive (p = 0.033) and PSWCs on EEG (p = 0.020) acted as the favorable and unfavorable factor for over 1 year of survival time, respectively.
Conclusions: There were some differences in epidemiological and clinical characteristics among patients in China and those of other countries. The prognosis and its influencing factors were relatively unexplored in China. The mean survival time of Chinese patients was longer than that of Caucasian patients but shorter than that of Japanese patients. The 14-3-3 protein in CSF and PSWCs on EEG were both closely related to the survival time. It is necessary to promote autopsy or biopsy to better understand sCJD in China.
Introduction
Prion diseases (PrDs), also known as transmissible spongiform encephalopathies (TSEs), are a group of rare, rapidly progressive and fatal central nervous system diseases attacking humans and animals. According to the modes of human PrDs, PrDs can be classified into three different categories: sporadic (spontaneous), genetic (familial, inherited), and acquired (infectious, transmitted). The sporadic type, the most prevalent one (85–90%), consists of sporadic Creutzfeldt–Jakob disease (sCJD), rare entities of sporadic fatal insomnia, and variably protease-sensitive prionopathy (VPSPr) (1, 2). sCJD is generally regarded as a spontaneous neurodegenerative illness, arising either from templated misfolding and protein conformation change of PrPC (normal brain prion-related protein) or spontaneous somatic mutation of PRNP (gene encoding the PrPC). sCJD is clinically characterized by rapidly progressive dementia with ataxia, myoclonus, or other neurologic symptoms (3, 4) and neuropathologically represented by the presence of aggregates of abnormal prion protein, spongiform change, neuronal loss, and gliosis (5). At least six molecular pathological subtypes are classified by genotypes of PRNP codon 129 and protease cleavage sites of PrPSc (misfolded disease-causing forms), which includes MM1, MV1, VV1, MM2, MV2, and VV2. They are responsible for the diversity of clinical characteristics like onset age, survival time, and symptoms (6, 7).
sCJD is a globally distributed disease. The identification of disease phenotype spectrum has greatly increased the possibility of early diagnosis of sCJD subtype specifically, which is also the foundation of developing effective treatments. In China, the CJD surveillance program supported by the Chinese Center for Disease Control and Prevention (CCDC) was conducted since 2006. sCJD cases (261) were reported nationwide from 2006 to 2010, with a higher number of cases in the east compared with those in other regions (8). However, the prevalence of sCJD has been rarely reported in the past decade in China. Besides, clinical features such as gender ratio and survival time are not consistent in several Chinese studies. Data on the prognosis of Chinese sCJD patients is even more deficient (8–14), and only one study had analyzed the factors affecting it (11). It is urgent to understand the current situation of sCJD in China. Here we presented a retrospective study on the epidemiologic and clinical characteristics and the prognoses of 67 sCJD patients in eastern China to raise awareness of this rare neurodegenerative disease.
Materials and Methods
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Discussion
In the current study, we retrospectively analyzed the demographic and clinical features of 67 sCJD patients in eastern China, and 43 of them received follow-ups. By the end of the study, 39 follow-up patients had died. Generally speaking, though PrDs are considered rare, the number of sCJD cases diagnosed in our hospital has been on the rise over the past 10 years. This may be attributed to the aging population, increasing number of patients admitted to hospital, and improved clinician awareness of the disease. However, the number of cases peaked in 2017 and has since declined mildly. It is for sure that the total number of inpatients from the department of neurology in our hospital has been increasing from 2010 to 2019. Given that the number of CJD cases nationwide in the recent 5 years has not been published, and other Chinese single-center study have not paid attention to the trend of the number of the patients, whether this trend is consistent with the national situation remains unknown.
In this study, we found that there were more female than male sCJD patients, and the female-to-male ratio was 1.39. Two studies of Chinese patients were consistent with our findings, in which the number of females predominated (10, 12), but two other studies had reported a higher proportion of males, where the female-to-male ratio was 0.82 and 0.79, respectively (8, 14). Because of small sample size and various nationalities in China, there might be biases in those results. A convincing gender ratio of sCJD patients in China requires demographic data of multicenter or population-based studies in the future. According to Japanese national sCJD surveillance data from 2001 to 2010, the ratio of female to male was 1.15 (16). In a research with 2,451 pathologically confirmed sCJD patients enrolled by the European Creutzfeldt–Jakob Disease Network (EUROCJD), which covered several European countries, Canada, and Australia, the ratio of female to male was 1.18 (7). In contrast, the data of 116 sCJD cases from the United States demonstrated that the female-to-male ratio was 0.49 (17). It is worth noting that this American study included patients of multiple races including Whites, Blacks, and American Indians. Race, population structure, molecular pathological subtype pattern, and the gender difference in economic status could partly contribute to the disparate ratios by countries. Further researches are required to investigate the exact reasons by controlling multiple factors. What is more, adequate sample size is indispensable in that the patient numbers of Chinese studies are much less than that in other countries.
The mean age of disease onset in this study (64.42 years old) was comparable with the findings from Korea (65.5 years) (18), Japan (65.5 years) (19), the United States (66.15 years), (20) and Europe (66 years) (21), but higher than that of a few Chinese patient groups [58 years (14) 60.3 years (9)]. Another two surveys in China have produced results essentially in agreement with ours at the median onset age, which was 64 years in our study (8, 11). According to these reported Chinese studies, most of suspected patients were not tested for PRNP gene to rule out genetic Creutzfeldt–Jakob disease (8, 9, 11, 14), which might affect the results and analysis of the study. Similarly, there were two patients younger than 30 years old in our study. Although these two patients met the criteria for clinical diagnosis of sCJD, they were not eventually diagnosed by pathological examination, which may also have an impact on the result. At the same time, considering that the different regional demographic features might play a role in the heterogeneity of onset ages among domestic studies, more accurate onset age remains to be verified by large national surveys. Additionally, Kotkowski et al. found that age (66 ± 11.5 years) was correlated with sCJD onset (20). As with many other neurodegenerative diseases, sCJD generally occurs in late adulthood.
So far, no effective treatment has been developed for sCJD, which remains uniformly fatal. In our study, the mean survival time was 9.39 months, and 72.09% of the sCJD patients had a survival time <1 year. Similarly, other researches in China showed that mean survival time ranged from 6.1 to 11.6 months, and 1-year mortality was 68.50–74.00% (11, 13, 14). We also found that 14-3-3 protein and PSWCs acted as the favorable and unfavorable factor for over 1 year of survival time, respectively. The same predictive effect of PSWCs was also observed in two European large-scale studies, in which over 2,000 pathologically definite sCJD cases were incorporated (7, 22). However, some studies have found that 14-3-3 protein was correlated with a shorter survival time (7, 21–24). The possible reasons for this discrepancy may be as follows: first of all, there are only two 14-3-3 protein-positive patients in this study, whose survival times were <1 year. Considering the small sample size and related uncertainties, our findings need to be further validated by large-scale studies. Second, the survival time is usually not determined by a single factor, which is why Llorens et al. have set up a multifactor predictive model (21). To our knowledge, previous study objects were Caucasians, but no Chinese researchers have explored the relationship between CSF biomarkers and survival time. Different races, regional environments, molecular subtypes and some relevant unknown factors may partly explain our different findings. Last but not the least, pathological examinations were not carried out in our patients, which could reduce the accuracy of diagnoses and then affect the results. Pocchiari et al. concluded that there was correlation between survival time and codon 129 type (22). The multinational study carried out by EUROCJD, whose research objects were mainly Caucasians, revealed that the mean survival time was only 5 months, and 85.8% of the cases died within 1 year. The MM, MV, and VV type of codon 129 accounted for 66.1, 17.0, and 16.9%, respectively (7). In Asia, almost all the sCJD patients were MM type (8, 9, 11, 14, 25–27). MM type accounted for 97.0–100.0% of all sCJD patients according to previous Chinese studies, while others were all MV type (8, 9, 11, 14, 27), which was in agreement with our findings. However, due to the extreme paucity of pathological examination, PrPSc types of Chinese sCJD patients are unavailable till now. As a consequence, different distribution patterns of PRNP codon 129 types may be one of the reasons for the variations in survival time, and other possible reasons remain to be explored.
However, according to a Japanese report, the mean survival time and 1-year mortality was 15.7 months and 48.1%, respectively (26). By comparison, survival time of the patients is shorter in China, where the MM type is the majority in Japan, though (8, 11, 14, 26). It should be noted that there is a proportion of Japanese patients with MM2 type, and nearly all of those survival time was more than 1 year, and some even reached 6 years (28). Additionally, the MM2 type can be further divided into MM2C and MM2T subtypes based on distinctive cortical and thalamic histopathology (2, 29), and the MM2T subtype is also known as sporadic familial insomnia (sFI), which is relatively rare. Their mean survival time was comparable despite different races (28, 30). Studies also have shown that MM1 type patients had an obviously shorter media survival time of 4.0–9.0 months (7, 31). However, the pathological classification of MM type is still not clear in China, which might be the main reason for the different survival time between Japanese and Chinese patients. More pathological results are needed to verify it. What is more, Iwasaki et al. found that tube feeding was an independent factor influencing total disease duration for MM1 type of sCJD (31), which also partly accounted for the longer survival time of Japanese patients (32). Future studies should be conducted by controlling possible interfering factors, such as regions, economic conditions, races, or molecular pathological subtypes, to find the exact factors that affect the course of disease.
The most common initial symptoms in our study were dementia, movement disorder, and visual disturbance, which were consistent with the results of other two centers in China (9, 11). It was reported by a Japanese research that 55.10% of cases started from psychiatric symptoms such as dementia or sleeping difficulties (26). According to a German study of 492 sCJD patients, dementia was the most common initial symptom followed by cerebellar, visual, and psychiatric disturbances (33). An American study with 114 sCJD patients showed that cognitive symptoms, especially memory loss, were the most common manifestations (34). Despite the discrepancies in race, region and even molecular pathological types, dementia is the most frequent onset symptom in these patients. Most notably, 49.25% of our patients rapidly developed progressive dementia, almost all of whom had this symptom at the time of seeking medical treatments.
The diagnosis of sCJD is challenging. Only autopsy can make a definite diagnosis, but most diagnoses of sCJD are based on clinical manifestations and ancillary tests without specific biological markers. Biomarkers with high specificity and sensitivity are urgently needed to assist clinicians in diagnosing sCJD. The first biomarker identified for diagnosis was PSWCs on EEG, which was performed in 63 patients in our study. Only 29 (46.03%) of them showed positive results, but the sensitivity of PSWCs among the confirmed CJD patients was 55.7–64% (7, 35). Since PSWCs usually occur in the late stages of the disease, the timing of test can also affect the positive rates. Serial EEG recordings are required for higher positive rates (36–38). Furthermore, given that PSWCs are common in encephalopathies, metabolic disorders, dementias, and other diseases, the specificity is relatively low (35, 39–41). Another widely used biomarker was 14-3-3 protein in CSF. As a moderately sensitive biomarker (42), 14-3-3 protein showed a positive rate of 68.00% in our cases, not very prominent detecting efficiency. Compared with PSWCs and 14-3-3 protein, brain diffusion-weighted MRI has a higher diagnostic utility in sCJD, with a sensitivity ranging from 92 to 96% and a specificity of about 93 to 94% (43–45). Further studies indicated that specific MRI abnormalities could serve as useful parameters for predicting the clinical course, such as those who have cortical plus basal ganglia hyperintensity on DWI tend to show symptoms of advanced stage (46, 47). The abnormal rate of brain MRI of our patients was 84.85%, which was a bit lower than that of studies with some defined patients (10, 42–44). One of the possible reasons is that our clinical diagnosis of sCJD is not conclusive without pathological inspections. In recent years, some of the newer diagnostic markers have also been explored; total (t)-tau protein in CSF was found to be superior to 14-3-3 protein and neurofilament light chain protein (NfL) as a biomarker with the highest sensitivity of 88.2–90.20% (48) and specificity of 74.5–78.9% (49, 50). The ratio of t-tau and phosphorylated-tau may be useful to distinguish patients with CJD from patients with other dementias (51, 52). In addition, a new PrPSc amplification assay, named real-time quaking-induced conversion (RT-QuIC), has also shown an overall diagnostic sensitivity of 82.1–92% and a specificity of 98.5–100% (49, 53–56), sometimes behind t-tau. Unfortunately, none of our patients had received the above two tests on account of technical limitations and examination costs. These problems could change with the deepening understanding and research of the disease.
Compared with nearly 100% in Austria (57), 68.7% in the United States (58), 66% in Germany (59), 60% in Australia (60), and 51% in Belgium (61), the autopsy rate is extremely low among Chinese patients due to the influence of traditional Chinese values. Besides, the fear and limited understanding of prion disease, as well as the restriction on the conditions of medical institutions, also affect autopsies on patients with this contagious neurodegenerative disease in China. Biopsy could not be performed smoothly and generally in China, either, which was confirmed by previous domestic literature (8, 10–13). Thus, their vast majority of molecular pathological subtypes remained unknown. To have a more accurate understanding of sCJD in China, the application of autopsy or biopsy in suspected patients is urgently needed.
This study described and analyzed the epidemiological and clinical characteristics of sCJD patients in eastern China systematically. Compared with most previous studies in China, we have gotten a relatively detailed clinical characteristics and complete follow-up data, and conducted a thorough analysis of the relationship between the two. Moreover, in the absence of autopsy and biopsy, compared with to the only study that performed prognostic analysis (11), the newer clinical diagnostic criteria were applied here. Differences in gender, 14-3-3 protein, PSWCs, and contributing factors on survival time were revealed in Chinese patients for the first time. There are also several limitations in our study. First, the number of sCJD cases was relatively small, and CSF 14-3-3 protein and PRNP gene analysis were not performed in all cases. With the ongoing deep research work, the rate of CSF and gene testing for patients with prion disease would increase gradually, thus, raising the accuracy of sCJD diagnosis. Second, some patients were lost to follow-up, resulting in unknown prognoses. In future research, we will expand the sample size and conduct more intensive and longer-term follow-up observations. Third, although the primary diagnostic methods for sCJD are t-tau and RT-QuIC, rather than the 14-3-3 protein and PSWCs now, the first two were not performed in this study. Finally, pathological examination of brain tissue, which is crucial to diagnosis, was not carried out. Along with the further understanding of prion disease and the development of medical and economic conditions in China, this situation is expected to be improved.
Conclusions
In conclusion, the epidemiological and clinical characteristics in Chinese studies are inconsistent, and there are some differences when compared with other countries. The mean survival time of Chinese patients was longer than that of Caucasian patients but shorter than that of Japanese patients. This study found that 14-3-3 protein in CSF and PSWCs on EEG acted as the favorable and unfavorable factor for over 1 year of survival time of Chinese patients, respectively, which has not been reported in previous literature of China. With an extremely low autopsy rate of prion disease in China, it is necessary to promote autopsy or biopsy to better understand the incidence, clinical characteristics, and the effective factors for prognosis of sCJD or prion diseases. Systematic studies on genes, protein, pathology, and clinical phenotypes are needed for a deeper insight of this disease as well as other possible neurodegenerative diseases related to protein misfolding.
https://www.frontiersin.org/articles/10.3389/fneur.2021.700485/full
83rd General Session • Paris, 24–29 May 2015 Final Report 2015
1. The Director General publish the following List of Member Countries recognised as having a negligible BSE risk in accordance with Chapter 11.4. of the Terrestrial Code:
Argentina Australia Austria Belgium Brazil Bulgaria Chile Colombia Croatia Cyprus Czech Republic Denmark Estonia Finland France Hungary Iceland India Ireland Israel Italy Japan Korea (Rep. of) Latvia Liechtenstein Luxembourg Malta Netherlands New Zealand Norway Panama Paraguay Peru Portugal Singapore Slovakia Slovenia Sweden Switzerland United States of America Uruguay
2. The Director General publish the following List of Member Countries recognised as having a controlled BSE risk in accordance with Chapter 11.4. of the Terrestrial Code:
Canada Chinese Taipei Costa Rica Germany Greece Lithuania Mexico Nicaragua Poland Spain United Kingdom
3. The Director General publish the following List of Member Countries having a zone4 recognised as having a negligible BSE risk in accordance with Chapter 11.4. of the Terrestrial Code:
China (People’s Rep. of): a zone designated by the Delegate of China in a document addressed to the Director General in November 2013, consisting of the People’s Republic of China with the exclusion of Hong Kong and Macau.
AND
4. The Delegates of these Member Countries shall immediately notify the OIE Headquarters if BSE occurs in their countries or their territories.
https://www.oie.int/app/uploads/2021/03/a-fr-2015-public.pdf
https://www.oie.int/app/uploads/2021/05/a-r17-2021-bse.pdf
https://www.nature.com/articles/srep11573
Prion diseases (PD) are the unique neurodegenerative proteinopathies reputed to be transmissible under field conditions since decades. The transmission of Bovine Spongiform Encephalopathy (BSE) to humans evidenced that an animal PD might be zoonotic under appropriate conditions. Contrarily, in the absence of obvious (epidemiological or experimental) elements supporting a transmission or genetic predispositions, PD, like the other proteinopathies, are reputed to occur spontaneously (atpical animal prion strains, sporadic CJD summing 80% of human prion cases).
Non-human primate models provided the first evidences supporting the transmissibiity of human prion strains and the zoonotic potential of BSE. Among them, cynomolgus macaques brought major information for BSE risk assessment for human health (Chen, 2014), according to their phylogenetic proximity to humans and extended lifetime. We used this model to assess the zoonotic potential of other animal PD from bovine, ovine and cervid origins even after very long silent incubation periods.
*** We recently observed the direct transmission of a natural classical scrapie isolate to macaque after a 10-year silent incubation period,
***with features similar to some reported for human cases of sporadic CJD, albeit requiring fourfold long incubation than BSE. Scrapie, as recently evoked in humanized mice (Cassard, 2014),
***is the third potentially zoonotic PD (with BSE and L-type BSE),
***thus questioning the origin of human sporadic cases.
We will present an updated panorama of our different transmission studies and discuss the implications of such extended incubation periods on risk assessment of animal PD for human health.
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***thus questioning the origin of human sporadic cases***
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***our findings suggest that possible transmission risk of H-type BSE to sheep and human. Bioassay will be required to determine whether the PMCA products are infectious to these animals.
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https://prion2015.files.wordpress.com/2015/05/prion2015abstracts.pdf
***Transmission data also revealed that several scrapie prions propagate in HuPrP-Tg mice with efficiency comparable to that of cattle BSE. While the efficiency of transmission at primary passage was low, subsequent passages resulted in a highly virulent prion disease in both Met129 and Val129 mice.
***Transmission of the different scrapie isolates in these mice leads to the emergence of prion strain phenotypes that showed similar characteristics to those displayed by MM1 or VV2 sCJD prion.
***These results demonstrate that scrapie prions have a zoonotic potential and raise new questions about the possible link between animal and human prions.
http://www.tandfonline.com/doi/abs/10.1080/19336896.2016.1163048?journalCode=kprn20
PRION 2016 TOKYO
Saturday, April 23, 2016
SCRAPIE WS-01: Prion diseases in animals and zoonotic potential 2016
Prion. 10:S15-S21. 2016 ISSN: 1933-6896 printl 1933-690X online
Taylor & Francis
Prion 2016 Animal Prion Disease Workshop Abstracts
WS-01: Prion diseases in animals and zoonotic potential
Transmission of the different scrapie isolates in these mice leads to the emergence of prion strain phenotypes that showed similar characteristics to those displayed by MM1 or VV2 sCJD prion.
These results demonstrate that scrapie prions have a zoonotic potential and raise new questions about the possible link between animal and human prions.
http://www.tandfonline.com/doi/abs/10.1080/19336896.2016.1163048?journalCode=kprn20
Title: Transmission of scrapie prions to primate after an extended silent incubation period)
*** In complement to the recent demonstration that humanized mice are susceptible to scrapie, we report here the first observation of direct transmission of a natural classical scrapie isolate to a macaque after a 10-year incubation period. Neuropathologic examination revealed all of the features of a prion disease: spongiform change, neuronal loss, and accumulation of PrPres throughout the CNS.
*** This observation strengthens the questioning of the harmlessness of scrapie to humans, at a time when protective measures for human and animal health are being dismantled and reduced as c-BSE is considered controlled and being eradicated.
*** Our results underscore the importance of precautionary and protective measures and the necessity for long-term experimental transmission studies to assess the zoonotic potential of other animal prion strains.
http://www.ars.usda.gov/research/publications/publications.htm?SEQ_NO_115=313160
Terry S. Singeltary Sr.